International Journal of Interdisciplinary Research
Variables | Coded levels for different enzymes | |||||
---|---|---|---|---|---|---|
Acid enzyme | Neutral enzyme | |||||
− 1 | 0 | + 1 | − 1 | 0 | + 1 | |
Concentration (%) | 0.5 | 1.25 | 2 | 0.5 | 1.25 | 2 |
Time (min) | 30 | 45 | 60 | 60 | 75 | 90 |
Mechanical agitation (number of steel balls) | 0 | 5 | 10 | 0 | 5 | 10 |